On - chip flow cytometry : where is it now & where is it going ? Editorial
نویسندگان
چکیده
Flow cytometry is a powerful, high-throughput, single-cell characterization and sorting tool, which has a unique capability to provide fast and quantitative analyses of individual cells and physical separation of cells of particular interest from other cells [1]. In flow cytometry, the cell analyses are performed by passing a narrow stream of cells through a focused laser beam at a rate of thousands of cells per second. Information regarding the size, type and content of cells can subsequently be derived through the analyses of the excited fluorescence emission or scattered light arising from each individual cell. In recent years, flow cytometry has rapidly become an indispensable instrument for many clinical diagnostics, ranging from routine blood tests to diagnoses of lethal diseases such as leukemia, respiratory infection and HIV/AIDS. Flow cytometry can screen multiple parameters and can be used for immunophenotyping. This has allowed diagnosis of diseases associated with B lymphocytes [1] and progress in the Human Immunology Project [2]. HIV is also typically monitored using flow cytometry [1]. Additionally, cell-sorting techniques have enabled flow cytometry to become a key tool for studying circulating tumor cells, which have potential in screening for and monitoring cancer [3,4]. With its extraordinary single-cell ana lysis and sorting capabilities, flow cytometry has been used extensively in numerous fields such as molecular biology, pathology, immunology, plant biology and marine biology. Despite their significant impact, current commercial f low cytometry systems have the following drawbacks: high cost, large instrumentation size and the constant requirement of highly trained personnel for maintenance (the latter two resulting from its complex system configuration).
منابع مشابه
Reflection for the undergraduate on writing in the portfolio: where are we now and where are we going?
Introduction: The portfolio can be seen as a tool for assessmentof a variety of learning activities that differ in content, usage, andassessment. The portfolio not only meets the learner’s educationalneeds but also the political and public reassurance demand thatthe health professional has achieved the required competency ofthe curriculum that allows him or her to practice safely with orwithout...
متن کاملI-36: Preimplantation Genetic Diagnosis - Where Have We Been and Where Are We Going
Preimplantation genetic diagnosis (PGD) is now considered routine in IVF laboratories with micromanipulation capability and access to genetic diagnostic services. The past two decades have witnessed a dramatic increase in the use of PGD, the number of cycles performed, and the indications for which PGD has been used. This increase has been mirrored by a slow, but steady, increase in the range o...
متن کاملAn Overview of Sex Selection at Conception in Mammals
Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...
متن کاملInduction of triploidy with caffeine treatment in the African catfish (Clarias gariepinus)
Induction of triploidy is one of the biotechnological methods in aquaculture used for genetic manipulation. It refers to a state where organisms have three complete sets of chromosomes instead of two and can result in sterility. Caffeine treatment that is safe and inexpensive, serve to induce triploidy in catfish. To suppress the second meiotic division, fertilized eggs were exposed to three di...
متن کامل